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Literature summary extracted from

  • Yu, W.; Wang, R.; Huang, H.; Xie, H.; Wang, S.
    Periplasmic nicotine dehydrogenase NdhAB utilizes pseudoazurin as its physiological electron acceptor in Agrobacterium tumefaciens S33 (2017), Appl. Environ. Microbiol., 83, e01050 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.99.4 expression in Escherichia coli Agrobacterium tumefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.99.4 0.00087
-
 (S)-nicotine cosubstrate 2,6-dichlorophenolindophenol, pH 7, 30°C Agrobacterium tumefaciens
1.5.99.4 0.00124
-
 2,6-dichlorophenolindophenol pH 7, 30°C Agrobacterium tumefaciens
1.5.99.4 0.00167
-
 (S)-nicotine cosubstrate pseudoazurin, pH 7, 30°C Agrobacterium tumefaciens
1.5.99.4 0.00361
-
 pseudoazurin pH 7, 30°C Agrobacterium tumefaciens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.99.4 periplasm
-
 Agrobacterium tumefaciens
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.4 Agrobacterium tumefaciens
-
-
-
1.5.99.4 Agrobacterium tumefaciens S33
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.99.4 purifiaction from the periplasmic fraction Agrobacterium tumefaciens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.99.4 22
-
 acceptor 2,6-dichlorophenolindophenol, pH 7, 30°C Agrobacterium tumefaciens
1.5.99.4 110
-
 acceptor pseudoazurin, pH 7, 30°C Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.4 (S)-nicotine + 2,6-dichlorophenolindophenol + H2O
-
 Agrobacterium tumefaciens (S)-6-hydroxynicotine + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.4 (S)-nicotine + 2,6-dichlorophenolindophenol + H2O
-
 Agrobacterium tumefaciens S33 (S)-6-hydroxynicotine + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.4 (S)-nicotine + pseudoazurin + H2O acceptor pseudoazurin is a single-electron carrier and is able to bind a single copper atom while accepting or discharging one electron Agrobacterium tumefaciens (S)-6-hydroxynicotine + reduced pseudoazurin
-
 ?
1.5.99.4 (S)-nicotine + pseudoazurin + H2O acceptor pseudoazurin is a single-electron carrier and is able to bind a single copper atom while accepting or discharging one electron Agrobacterium tumefaciens S33 (S)-6-hydroxynicotine + reduced pseudoazurin
-
 ?
1.5.99.4 additional information NAD(P)+, O2, and ferredoxin cannot serve as electron acceptors Agrobacterium tumefaciens ?
-
-
1.5.99.4 additional information NAD(P)+, O2, and ferredoxin cannot serve as electron acceptors Agrobacterium tumefaciens S33 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
1.5.99.4 NdhAB
-
 Agrobacterium tumefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.99.4 7
-
 acceptor pseudoazurin Agrobacterium tumefaciens

General Information

EC Number General Information Comment Organism
1.5.99.4 physiological function disruption of the gene encoding pseudoazurin disables nicotine degradation Agrobacterium tumefaciens